Reserve Matches
By Total Tranmere
March 19 2010
| Date | Opponent | At | Score/KO | Scorers/Location |
| 9th Sep | Walsall | H | W 2-1 | Kirby, Jago (Pen) |
| 22nd Sep | Morecambe | A | L 4-3 | G.Donnelly, Rowe, Holness |
| 8th Oct | Fleetwood Town (DLCGD) | A | L 3-1 | Illesanmi |
| 14th Oct | Shrewsbury Town | H | D 2-2 | Kirby x 2 |
| 28th Oct | Wigan Athletic | A | D 3-3 | Moynes, Rowe (pen), Richards |
| 12th Nov | Morecambe (DLCGD) | H | L 3-0 | |
| 22nd Dec | Wigan Athletic (DLCGD) | A | L 4-0 | |
| 6th Jan | Bootle (LSC) | A | L 2-1 | G.Donnelly |
| 13th Jan | Nantwich Town (CSCQF) | A | W 5-1 | G.Donnelly, Kirby, Moynes, Richards x 2 |
| 27th Jan | Port Vale | H | L 2-1 | G.Donnelly |
| 3rd Feb | Wigan Athletic | H | L 1-0 | |
| 17th Feb | Northwich Victoria (CSCSF) | A | L 1-0 | |
| 3rd Mar | Wrexham | H | D 1-1 | Omotola |
| 10th Mar | Port Vale | A | 2pm | at TBC |
| 25th Mar | Wrexham | A | 1pm | at TBC |
| 31st Mar | Morecambe | H | 2pm | at Vauxhall Motors FC |
| 8th Apr | Shrewsbury Town | A | 2pm | at Shrewsbury Sports Village |
| 28th Apr | Walsall | A | 2pm | at Chasetown FC |