There are three changes with Dan George repacingt injured hooker Darren Dawidiuk and James Hudson returning in the second row in place of Will James.
Twelvetrees replaces Ryan Mills who was injured at Exeter Chiefs last Saturday and Mike Tindall moves across to inside centre alonsgide Henry Trinder.
Gloucs: Rob Cook, Charlie Sharples, Henry Trinder, Mike Tindall, Jonny May, Billy Twelvetrees (c), Jimmy Cowan, Nick Wood, Dan George, Shaun Knight, Elliott Stooke, James Hudson, Sione Kalamafoni, Matt Kvesic, Ben Morgan
Reps: Huia Edmonds, Yann Thomas, Sila Puafisi, Will James, Gareth Evans, Tavis Knoyle, Freddie Burns, Martyn Thomas
Wasps welcome back Joe Launchbury and Matt Mullan and on the wings James Short replaces Tom Howe and Will Helu, who has recovered from injury, replaces Jonah Holmes.
Wasps: Andrea Masi, Will Helu, Elliot Daly , Chris Bell (c), James Short , Andy Goode, Joe Simpson, Matt Mullan, Carlo Festuccia , Jake Cooper-Woolley, Joe Launchbury, Kearnan Myall, Sam Jones, James Haskell , Nathan Hughes.
Reps: Tom Lindsay, Simon McIntyre, Phil Swainston, Tom Palmer, Guy Thompson, Charlie Davies, Joe Carlisle, Charlie Hayter
